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年度	112	專案性質	實驗性質
專案類別	研究專案	研究主題	調查
申請機構	國立中正大學	申請系所	地球與環境科學系
專案主持人	許昺慕	職等/職稱	特聘教授
專案中文名稱	整合泥火山與生物炭於微生物燃料電池系統進行三氯乙烯地下水污染生物整治
中文關鍵字	微生物燃料電池, 生物刺激, 泥火山, 微生物菌群, 三氯乙烯
專案英文名稱	Integrating Volcanic Mud and Biochar in MFC Systems for the Bioremediation of TCE Contaminated Groundwater
英文關鍵字	Microbial Fuel Cell (MFC), Biostimulation, Mud Volcanoes, Microbiota, Trichloroethylene (TCE)
執行金額	975,822元
執行期間	2023/12/15 至 2024/11/29
計畫中文摘要	三氯乙烯 (TCE) 因其具有持久性和毒性，當其污染地下水會對環境和健康構成重大風險。因此，開發有效的技術以進行受污染地下水之整治至關重要。本研究乃評估以整合生物炭的管柱微生物燃料電池 (TMFCs) 用於 TCE 污染地下水整治之可行性，並透過甲烷氧化菌之接種進行強化。研究結果顯示，在實驗室規模的處理組 (T) 中，與無生物炭的處理組 (TWB組) 和對照組 (C) 相比，T 組的 TCE 降解能力有所提升，而 TWB 和 C 組的 TCE 降解不完全。T 組中，Methylococcus capsulatus、Hydrogenophaga pseudoflava、Dehalococcoides mccartyi、Geobacter sulfurreducens 和 Desulfovibrio oxamicus 的豐度顯著增加，特別是在培養時間為15天、30天 和45天的樣本中，而 TWB 組中則次之。相比之下，C 組中，以Methanococcus jannaschii、Methanobacterium subterraneum 和 Brevundimonas diminuta豐度較為突出。qPCR 結果顯示，T 組中與甲烷氧化 (mmoX、mmoA)、電子轉移 (omcX) 和還原脫氯 (rdhA、tceA、vcrA) 相關的功能基因豐度顯著增加。相比之下，C 組中與甲烷生成活性相關的mcrA基因量較高。在現地管柱微生物燃料電池中，我們觀察到相似的模式，特別是在90天樣本中甲烷氧化 (mmoX、pmoA)、電子轉移 (omcX) 和還原脫氯 (rdhA、tceA、vcrA) 基因顯著增加，mcrA基因量較低，顯示甲烷生成活性顯著降低。此外，硫酸鹽、鐵和錳的代謝在 T 組中較 TWB 和 C 組更加活躍。在還原條件下，管柱微生物燃料電池展現了良好的分解效果，其水質參數條件分別為: pH值為 7.5、溫度為 25.2°C、氧化還原電位(ORP)為-517 mV。實驗室規模的管柱微生物燃料電池展示了對 TCE 生物修復的有效性，也突顯了以整合生物炭和甲烷氧化菌群的管柱微生物燃料電池作為TCE污染地下水整治是環保且永續的解決方案。
計畫英文摘要	Trichloroethylene (TCE) contamination in groundwater poses a significant environmental and health risk due to its persistence, and toxicity. Therefore, it is important to develop effective technologies to remediate contaminated groundwater. This study evaluated biochar-integrated tubular microbial fuel cells (TMFCs) for TCE-contaminated groundwater remediation, enhanced by methanotrophic inoculation. Results showed that in the lab-scale treatment (T) group had improved TCE degradation compared to treatment without biochar (TWB) and control (C) groups, which showed incomplete TCE degradation. The T group had significantly higher abundances of Methylococcus capsulatus, Hydrogenophaga pseudoflava, Dehalococcoides mccartyi, and Geobacter sulfurreducens and Desulfovibrio oxamicus and second most abundant in TWB group particularly evident on D-15, D-30, and D-45. Whereas, Methanococcus_jannaschii, Methanobacterium_subterraneum and Brevundimonas diminuta were prominent in C group. qPCR analysis revealed significantly higher abundances of functional genes associated with methane oxidation (mmoX, mmoA), electron transfer (omcX), and reductive dechlorination (rdhA, tceA, vcrA) in the T group. In contrast, the C group exhibited higher levels of the mcrA gene associated with methanogenic activity. In the in-situ (IS) TMFC reactor, a similar pattern was observed, with significant increased in methane-oxidizing (mmoX, pmoA), electron transfer (omcX), and reductive dechlorination (rdhA, tceA, vcrA) genes, particularly at D-90. This reactor exhibited low mcrA gene levels, indicating a noticeable decreased in methanogenic activity in the reactor. Additionally, sulfate, iron, and manganese metabolism were highly active in T group compared to TWB and C groups. The TMFC reactors showed promising results under reducing conditions, with a pH of 7.5, a temperature of 25.2°C, and an oxidation-reduction potential (ORP) of -517 mV. The TMFCs demonstrated effectiveness for TCE bioremediation, highlighting the potential of biochar and methanotrophic consortia integrated TMFCs as an eco-friendly and sustainable solution for TCE-contaminated groundwater remediation.