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年度	109	專案性質	實驗性質
專案類別	模場試驗	研究主題	整治
申請機構	國立中央大學	申請系所	生命科學系
專案主持人	陳師慶	職等/職稱	教授
專案中文名稱	開發高效脫氯生物膠體及優化生物包埋整治工法：現地模場試驗
中文關鍵字	脫鹵球菌,生物膠體,氯乙烯,完全脫氯
專案英文名稱	Develop high-efficiency dechlorination bio-gel and optimize the immobilized strategy of bioremediation in chlorinated contamination: pilot study
英文關鍵字	dehalococcoides, bio-gel, vinyl chloride, completed dechlorination
執行金額	2,300,000元
執行期間	2020/1/1 至 2021/11/30
計畫中文摘要	三氯乙烯含氯有機物因其特性為比水重之非水相液體(dense non-aqueous phase liquid, DNAPL)，進入地下環境後，因其低黏滯性使其可在地表下快速移動，低表面張力使其容易穿入土壤小孔隙或岩石裂隙，造成整治困難度高。生物整治法被喻為自然、環保及節能的處理技術，也是目前環境污染整治的趨勢。現地生物復育技術可行性其中最重要條件之一為場址本身須具有可降解污染物之微生物。當場址中可降解污染物微生物數量較低或場址整治急迫性高時，可額外添加微生物進行生物強化(Bioaugmentation)整治受污染之場址。雖然許多微生物已證明可降解TCE至中間產物 Vinyl chloride (VC)，但VC具有更高毒性和累積性，因此如何去除環境中 VC 污染，是生物復育一項重要課題。本研究第一年度欲建構絕對厭氧操作技術，大量培養脫鹵球菌，使得菌量達可以達應用於現地整治之標準，延伸開發本土脫氯共生菌群，同時開發高效脫氯生物膠體，包埋脫鹵球菌與產氫菌，結合污染攔截牆工法，有效提升生物基質之利用。本團隊所培養的純種脫鹵球菌可以將80mole在9天內完全降解成無毒的乙烯。經優化後之脫氯膠體，可成功包埋產氫菌及脫鹵球菌於膠體之中，並且確認本膠體可以長時間使用長達183天以上，並且可以維持脫鹵球菌及產氫菌之活性，膠體可以重複使用至少26次以上，因此具有現地應用之潛力。
計畫英文摘要	Chlorinated organic compounds such as trichloroethene (TCE), which characteristic is dense non-aqueous phase liquid (DNAPL). When entering the underground environment, it can move rapidly under the surface due to its low viscosity, and its low surface tension makes it easy to penetrate the soil small pores or rock cracks, causing remediation difficult. Bioremediation has been hailed as a natural, eco-friendly and energy-saving treatment technology, and also the current trend of environmental pollution remediation. One of the most important conditions for on-site bioremediation technology is that the site itself must have microbes that can degrade contaminants. When the number of the degradable microbes at the site is low or the remediation site is of high urgency, additional microorganisms may be added for bioaugmentation to remediate contaminated sites. Although many microorganisms have been shown to degrade TCE to the intermediate product Vinyl chloride (VC), VC is more toxic and cumulative, so how to remove VC contamination in the environment is an important issue in biological breeding. In the first year of this study, we wanted to construct an absolute anaerobic operation technology, and cultivate a large number of dehalococcoides, so that the amount of bacteria can reach the standard of on-site remediation, extend the development of local dechlorination symbiotic bacteria, and develop high-efficiency dechlorination bio-gel. Dehalococcoides and hydrogen-producing bacteria are combined with the pollution intercepting wall method to improve the utilization of biological substrates effectively.