成果專區 - 土壤及地下水污染整治基金補助研究與模場專案宣導網

專案基本資料

年度	108	專案性質	實驗性質
專案類別	模場試驗	研究主題	整治
申請機構	國立中央大學	申請系所	生命科學系
專案主持人	陳師慶	職等/職稱	教授
專案中文名稱	應用產氫菌提升產氫量以加強含氯有機污染物還原脫氯效率：現地模場試驗
中文關鍵字	氯乙烯,生物強化,產氫菌,脫氯菌,菌株固定化
專案英文名稱	Application of hydrogen-producing bacteria to enhance reductive dechlorination efficiency of chlorine ethene : field test
英文關鍵字	Vinyl chloride,Bioaugmentation, hydrogen-producing bacteria,dechlorination bacteria,immobilizatio
執行金額	1,200,000元
執行期間	2019/1/1 至 2019/11/30
計畫中文摘要	含氯有機物因其特性為比水重之非水相液體(dense non-aqueous phase liquid, DNAPL)，進入地下環境後，因其低黏滯性使其可在地表下快速移動，低表面張力使其容易穿入土壤小孔隙或岩石裂隙，造成整治困難度高。生物整治法被喻為自然、環保及節能的處理技術，也是目前環境污染整治的趨勢。在厭氧環境，已被證實一種脫氯菌 (Dehalococcoides)可以完全還原VC至乙烯，但脫氯作用需要氫氣作為電子提供者，降解初期氫氣含量仍充足，因此脫氯作用速率較為快速，但至降解後期氫氣逐漸消耗殆盡使脫率速率降低。研究期末報告部分，對其進行運轉中菌相調查，包埋產氫菌膠體結合乳化油之生物刺激法，於反應井中菌相組成穩定，可以測得發酵者Clostiridium spp.及Desulfovibrio spp.，以及提共維他命B12之相關菌株Desulfovibrio spp.及Methanosarcina sp.，以qPCR分析地下水樣本，反應井之包埋產氫菌組別測得其脫鹵球菌菌數可以長時間維持再105/L，且相關功能性基因如tceA、vcrA及bvcA皆有所提升，VC RDase與脫鹵球菌總菌數之比例維持在0.3~3之間可以觀測到乙烯之產生，證實生物產氫膠體對於脫鹵球菌菌數及功能性基因的表現影響相當有效果。本計畫成功利用生物包埋產氫菌達成現地地下水試驗，經過添加生物包埋產氫菌則可以提升脫鹵球菌菌數及功能性基因的表現，進而增強降解多氯乙烯達成完全降解至乙烯之功效，確實降解現地污染物cis-DCE，並在反應井中測得乙烯之產生。
計畫英文摘要	Chlorine-containing organic matter is dense non-aqueous phase liquid (DNAPL) because of its characteristics, and enters the underground environment. DNAPL has low viscosity, it can move rapidly under the surface. It is easy to penetrate into small pores or cracks in the soil, resulting in high difficulty in remediation. The bioremediation method has been hailed as a natural, environmental, and energy-saving treatment technology, and it is also a current trend of environmental pollution remediation. In an anaerobic environment, it has been confirmed that Dehalococcoides spp. can completely reduce VC to ethylene, but Dechlorination spp. requires hydrogen as an electron supplier. The hydrogen content is still sufficient in the initial stage of degradation, so the rate of dechlorination is relatively fast, but at the end of the degradation stage, hydrogen is gradually depleted and the rate of depletion is reduced. At the end of the research report, the immobilized hydrogen-producing bacteria gel combined SPRS was applied in the pilot study. By the metagenome analysis, the composition of microcosm in the reaction well was stable. We can found Clostridium spp. and Desulfovibrio spp. as fermentors. Methanosarcina sp., which is a strain that can supply vitamin B12. qPCR used to analyze groundwater samples. The number of Dehalococcoides in the reaction wells was maintained at 105/L for a long time. Functional genes, such as tceA, vcrA, and bvcA were improved. The ratio of VC RDase to the total number of Dehalococcoides was maintained between 0.3 and 3. Ethylene production was observed, and the number of Dehalococcoides was confirmed. And the immobilized hydrogen-producing bacteria gel performance effects of functional genes are quite practical. The project successfully used the bio-encapsulated hydrogen-producing bacteria to reach the groundwater test in situ. After adding the bio-encapsulated hydrogen-producing bacteria, the number of Dehalococcoides and functional genes can be improved, and the degradation of polyvinyl chloride can be enhanced to achieve complete degradation to ethylene. The effect is that the local pollutant cis-DCE is degraded, and the production of ethylene is measured.

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