跳到主要內容 :::

年度	108	專案性質	非實驗性質
專案類別	研究專案	研究主題	調查
申請機構	國立臺灣大學	申請系所	食品安全與健康研究所
專案主持人	陳玟伶	職等/職稱	助理教授
專案中文名稱	以高解析質譜法篩檢施灌沼液之農地土壤及農作物中的動物用藥品
中文關鍵字	沼液,土壤,農作物,動物用藥品,高解析質譜
專案英文名稱	Screening vet drugs in the soil and crops grown in farmlands irrigated with biogas slurry using high-resolution mass spectrometry
英文關鍵字	high-resolution mass spectrometry, livestock wastewater, biogas slurry, crops, vet drugs, food safety
執行金額	649,818元
執行期間	2019/1/1 至 2019/11/30
計畫中文摘要	畜牧糞尿經過厭氧發酵所產生之沼液施灌於農地，不僅能做為農地肥份，還可改善水污染問題；然而沼液中的動物用藥品是否因此進入農地環境、累積於農地土壤、甚至影響食品安全，仍存在許多未知。本研究透過定性分析，篩選可能因灌溉沼液而進入農地土壤、被農作物吸收的動物用藥品。本研究以二座養豬畜牧場（C與U）及其自有農地為研究對象，使用高解析質譜儀快速掃描、獲取、紀錄畜牧廢水原水、沼液、土壤、地下水、及農作物樣品中的化合物準確質量，並與動物用藥品資料庫中2,701種藥品與代謝物比對。結合2019年3、5、7月採樣分析結果發現，應用高解析質譜法能在所有樣品中成功篩檢出動物用藥品與代謝物。在2,701種動物用藥品中，C畜牧場樣品總共比對出116種，U畜牧場有79種；且U畜牧場的每一種樣品中所比對出的藥品種類均少於C畜牧場。我們觀察藥品種類，得知長期使用多重動物用藥品，使抗生素、鎮靜安眠藥物、解熱鎮痛藥物、局部麻醉藥物、合成代謝藥物廣泛存在畜牧場環境，其中抗生素、抗寄生蟲藥、解熱鎮痛藥物會滯留於農地土壤，而其中水楊酸類藥品會被農作物吸收，從上澆灌沼液則會使合成代謝藥物norethindrone acetate吸附在農作物食用部位。本研究在不介入畜牧業者既有操作管理行為的前提下，呈現了畜牧場、農地環境、農作物中動物用藥品長期多批混合、滯留、分布之全貌，歸納出殘留在農地土壤與農作物中的藥品種類。建議後續計畫以本研究在土壤與農作物中定性篩檢出的動物用藥品與代謝物為目標物，進行多重動物用藥品與代謝物的定量分析，作為未來環境與健康風險評估、藥品管理、改進廢水處理之參考。
計畫英文摘要	The irrigation using biogas slurry produced by anaerobic fermentation of livestock urine can fertilize the farm and mitigate water pollution. However, much is still unknown regarding vet drugs in biogas slurry, the accumulation of these chemicals in the soil, and the following food safety problems. This study used a qualitative method to screen vet drug residues that enter the soil of farmlands and agriculture products through biogas slurry irrigation. We studied two pig farms (Farm C and U) and their agricultural farms. A high-resolution mass spectrometer was used to rapidly scan, acquire, and record the accurate masses of compounds in the pig urine, biogas slurry, soil, groundwater, and crops. The accurate masses were then compared to those of over 2,701 vet drugs in the databases. The analytical results of the samples grabbed in March, May and July 2019 revealed that application of high-resolution mass spectrometry succeeded in the screening of vet drugs and metabolites in each sample. A total of 116 and 79 vet drug matches were present in the samples taken from Farm C and U, respectively. In addition, the number of matches in each sample type was lower in Farm U than that in Farm C. Long-term use of multiple vet drugs results in the ubiquity of antibiotics, sedative and hypnotic drugs, antipyretic and analgesic drugs, local anesthetics, and anabolic drugs in livestock farm environments; among these drugs, antibiotics, antiparasitic drugs, antipyretic and analgesic drugs may retain in the soil. Moreover, salicylic acid drugs can be absorbed by the crops. Watering the biogas slurry from above will cause the adsorption of an anabolic drug (norethindrone acetate) onto the edible parts of crops. Under the premise of not intervening in the operation and management behavior of the animal husbandry, this study presents the long-term multi-batch mixing, retention, and distribution of vet drugs in livestock farms, farmland environment and agriculture products. We suggest that the future projects may target on the vet drugs we screened out in the soil and crops, conducting the quantitative determination of multiple drug residuals for further environmental and health risk assessments, drug management, and wastewater treatment improvement.