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年度	107	專案性質	實驗性質
專案類別	研究專案	研究主題	整治
申請機構	國立屏東科技大學	申請系所	環境工程與科學系
專案主持人	趙浩然	職等/職稱	教授
專案中文名稱	應用電化學氧化法及生物毒性篩檢技術於受含氯乙烯及苯類污染地下水之現地整治可行性研究
中文關鍵字	電化學氧化法,生物毒性篩檢技術,氯乙烯,苯類污染物,地下水
專案英文名稱	Feasibility study on electrochemical oxidation and high-throughput toxicity screening assays for the remediation of vinyl chloride and phenyl compounds contaminated groundwater
英文關鍵字	Electrochemical oxidation process,groundwater
執行金額	920,000元
執行期間	2018/1/10 至 2018/11/30
計畫中文摘要	電化學高級氧化法(electrochemical advanced oxidation process)為一種高級氧化技術(advanced oxidation processes, AOPs)，具有高效節能、操作簡單、環保及設備簡單，其中又以BDD (boron-doped diamond)做為陽極對於污染物之去除效率較高，相較於其它材料具有低成本與較高之污染物礦化效率等優點。本研究以國外電極BDD與國內電極BDD降解含氯乙烯類及苯類污染物，共採集10件次受苯類污染及6件受氯乙烯類污染之地下水樣本。本次研究結果顯示經電化學處理後之MTBE、Chlorbenzene、Ethylbenzene、1,3-Dichlorobenzen、1,2- Dichlorobenzene、1,4- Dichlorobenzene、Trichloroethene、cis-1,2-dichloroethene及Trichloroethene等污染物濃度均下降至符合法規標準，而m,p-Xylene及o-Xylene於第一季電解2小時試驗中之去除率僅69.4%與87.5%；但其在第二季電解4小時試驗之去除效率可達到90.3%及99%，意味著增加電解時間可有效提升上述污染物之去除效率。進一步使用國內(SW03-D-S2)與國外(SW03-I-S2)電極分別進行4小時的電化學試驗，總有機碳去除效率為95%，且苯類污染物濃度皆符合第二類地下水污染物管制標準限值。地下水樣本進行電化學氧化處理後，搭配三種生物毒性篩測平台(綜合細胞毒性分析(Cytotoxic ATP bioassay)、致癌毒性分析(P53 bioassay)與發炎反應生物偵測法(NF-κB bioassay))，評估整治後之生物毒性，進一步探討整治效益與毒性風險評估。本研究p53 bioassay和NFκB bioassay分析結果顯示，處理後之樣本（230 ng-DOX / L和534 ng-TNFα/ L）毒性效應均顯著低於未處理樣本（657.5 ng-DOX / L和26625 ng-TNFα/L）。而在ATP biosassay結果發現處理後的樣品（84.5％）相較於處理前樣品（80.2％）之細胞存活率較高，但兩者並沒有觀察到顯著差異(p>0.05)。本土開發之BDD電極用於處理污染之地下水有機污染物具有良好之潛力，且此整治方法可顯著改善受污染地下水對人體毒性及生態風險之影響。
計畫英文摘要	The electrochemical advanced oxidation process, one of advanced oxidation processes (AOPs) for contaminant removal, relies heavily on anode material production technology. Compared with other materials, the boron-doped diamond (BDD) anode has advantages which include low cost and high mineralization efficiency of contaminants. The aim of this study is to use locally-produced and commercial BDD anode material to remediate groundwater contaminated with vinyl chloride and phenyl compounds. Ten and six groundwater samples were collected from phenyl and vinyl chloride compounds-contaminated groundwater area in Southern Taiwan in this study. After electrochemical oxidation treatment, most of contaminants including MTBE, Ethylbenzene、1,4-Dichlorobenzene, 1,2-Dichlorobenzene Trichloroethene, cis-1,2-dichloroethene, and Trichloroethene were completely degraded in the whole samples. However, low degradation rates of m,p-Xylene (69.4%) and o-Xylene (87.5%) were observed in this study in which the treatment time was 2 hours. While the treatment time extend to 4 hours, m,p-Xylene and o-Xylene degradation rates were improved to 90.3% and 99.0%. Compared between locally-produced (SW03-D-S2) and commercial (SW03-I-S2) BDD anodes, the high degradation rates of total organic carbon and phenyl contaminants occurred in both BDD anodes and said levels of contaminants were lower than the standard values. Three high-throughput toxicity screening assays (HIT assay) for the carcinogenicity pathway (p53 bioassay), cytotoxicity (Cytotoxic ATP biosassay), and the inflammation response (NFκB bioassay) were used to screen for the toxicity of the remediated groundwater samples and do further toxicity risk assessment. We observed that the average toxicity effects of p53 bioassay and NFκB bioassay in treated samples (230 ng-DOX/L and 534 ng-TNFα/L) were significantly lower than untreated samples (657.5 ng-DOX/L and 26625 ng-TNFα/L). The ATP bioassay result showed that there is a high cell survival rate for the treated samples (86.5%) as compared to untreated samples (80.2%). In conclusion, the lab-made BDD anode has great potential to remediate groundwater contaminated with organic compounds and this remediation technique can significantly allivate the adverse effects of toxicity on human health and ecological risk.