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年度	107	專案性質	實驗性質
專案類別	研究專案	研究主題	整治
申請機構	國立成功大學	申請系所	環境工程學系
專案主持人	吳哲宏	職等/職稱	副教授
專案中文名稱	地層多氯乙烯汙染精準生物復育技術:建立脫氯菌生態網絡
中文關鍵字	三氯乙烯, 精準生物復育, 脫率菌, 生態網絡
專案英文名稱	Precision bioremediation of trichloroethylene in groundwater: Build up microbial ecological network
英文關鍵字	trichloroethylene, Precision bioremediation, microbial ecological network
執行金額	1,020,000元
執行期間	2018/1/10 至 2018/11/30
計畫中文摘要	三氯乙烯厭氧還原脫氯的去毒化過程常發生毒性更高之二氯乙烯或氯乙烯代謝物累積。為發展三氯乙烯完全脫氯的生態控制技術，本研究建立實驗室規模厭氧還原脫氯反應系統，探討添加不同藥劑化合物(糖蜜、乳化油與乙酸) 的條件下對三氯乙烯還原脫氯反應及微生物體結構的影響。本研究操作三個添加不同藥劑的反應槽，利用含碳量、酸類、乙烯及多氯乙烯濃度變化來評估三個反應槽TCE還原脫氯之功能表現，結果顯示添加的三種藥劑碳源均能促進TCE完全脫氯成乙烯，但是在高TCE負荷的條件下，乙酸作為碳源的反應槽中乙酸降解菌群活性受到抑制，使得完全脫氯效率低於其他反應槽，並累積較高濃度的二氯乙烯。高通量定序分析菌相的結果顯示，添加不同複雜程度的碳源均導致整體的菌群結構改變，尤其在添加糖蜜的情況下，微生物多樣性最為豐富，但不同的碳源並不會明顯地改變脫氯菌的種類及豐富度，也因此三個使用不同碳源的反應槽其TCE還原脫氯速率不相上下。另一方面，分析脫氯菌群與其他微生物網絡關係發現結構簡單的碳源，如乳化油與乙酸，其物種互動關係是相當複雜的，且菌群結構隨時間變動劇烈。相對地，使用結構較複雜的糖蜜作為碳源，微生物菌群結構較穩定，脫氯菌群與其他微生物的網絡關係也較為簡單。本研究建立了藥劑對脫氯微生物體與TCE脫氯表現的關聯性，相關成果有助於發展本土地下水TCE污染精準生物整治技術。
計畫英文摘要	Under anaerobic conditions, the detoxification process of trichlorethylene often results in the accumulation of dichloroethylene or vinyl chloride metabolites, which are more toxic than trichlorethylene. In order to develop an ecological control technology for the complete dechlorination of trichlorethylene to ethylene, this study is aimed to establish the laboratory-scale anaerobic reductive dechlorination systems to investigate effects of the three hydrogen-releasing agents (i.e., molasses (MS), emulsified oil (EOS) and acetate (AS)) on the performance of reductive trichlorethylene dechlorination, and the corresponding microbiome structure. In this study, the dechlorination performance of three reactors was evaluated by analyzing concentrations of organic carbon, volatile acids, and chlorinated ethylene. The results showed that all of the reagents can stimulate the complete dechlorination of TCE. However, under the condition of high TCE loading rate (26.4 mg TCE/L/day), the acetate-degrading microbial populations in the acetate-fed reactor was inhibited, leading to a greater accumulation of cDCE and lower efficiency of complete dechlorination, as compared with the MS and EOS reactors. The results of high- throughput sequencing analysis of 16S rRNA gene showed that although the reagents possess different carbon complexity, the overall microbiome structures of the reactors varied differently. In the case of adding molasses, the microbial diversity was the most abundant. However, different carbon sources would not significantly change the type and richness of dechlorinating bacterial populations, which is consistent with the performance of TCE reduction and complete dechlorination among three reactors. In addition, the analysis of the interactive relationship between dechlorinating bacterial groups and other microbial populations reveals that simple carbon sources, such as emulsified oil and acetic acid, have a complex interaction network with species, and the structure of the flora changes drastically with time. In contrast, with the use of more complex carbon source such as molasses, the structure of the microbial flora is relatively stable, and the ecological network of the dechlorinating groups is relatively simple. This study established the associations of different agents, their complete dechlorination capability, and the resulting microbiomes. The overall results provide useful information to facilitate the development of precision bioremediation technology for groundwater TCE pollution in Taiwan.