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年度	99	專案性質	實驗性質
專案類別	研究專案	研究主題	整治
申請機構	國立雲林科技大學	申請系所	環境與安全衛生工程系
專案主持人	林啟文	職等/職稱	教授
專案中文名稱	新穎釋氧物質受油品污染之地下水整治與Microtox生物毒性評估技術研發-批次與管柱試驗
中文關鍵字	生物毒性；固定化菌體顆粒；活化過硫酸鹽氧化
專案英文名稱	Development of a novel oxygen-releasing material and microtox toxicity evaluation for gasoline-contaminated groundwater–Batch and column experiments
英文關鍵字	Biotoxixity；cell-immonilized bead；activated persulfate oxidation
執行金額	0元
執行期間	2010/12/29 至 2011/12/28
計畫中文摘要	"本計畫主要利用生物毒性偵測法Vibrio fischeri light inhibition test方法，檢測地下水污染物生物毒性範圍，若地下水具高毒性水質則採用化學法方式進行整治；若具中低毒性則以生物法方式進行復育。地下水整治方法選擇係採用釋氧型生物法與活化過硫酸鹽氧化法等二種方式進行。其中，「化學法」採用不同低價吸附劑(高爐石、轉爐石)種類與劑量，以異相活化過硫酸鹽方式進行對BTEX降解效率評估。「生物法」則以生物可分解之固化材料(聚乙烯醇)，將可降解油品污染物之分解菌種(BTEX分解菌)及釋氧物質(過氧化鈣)共同包埋而形成釋氧型固定化菌體顆粒。
計畫英文摘要	"The objective of the project was to establish a framework for the integration remediation in a contaminated groundwater. First, the bioassay, Vibrio fischeri light inhibition test, was used to determine the toxicity of petroleum-contaminated groundwater. The highly toxic groundwater was treated with chemical oxidation method. In contrast, biological method was applied for groundwater of moderate-to-low toxicity. The oxygen-releasing type for a biological method and activated persulfate oxidation were selected to remediate a BTEX-contaminated groundwater. For the biological method, novel immobilized beads for oxygen releasing were manufactured by incorporating calcium peroxide (CaO2), with BTEX-degrading bacteria using a biodegradable material composed of polyvinyl alcohol (PVA) and alginate. For the chemical method, two types of adsorbents (blast-furnace slag (BF slag), blast oxygen furance slag (BOF slag)) were used to activate sodium persulfate. Moreover, batch or column tests were conducted to investigate the amounts of beads and concentrations of sodium persulfate and adsorbents on BTEX decomposition for biological and chemical methods, respectively. Both BF and BOF slags show the capability in activating sodium persulfate leading to the biodegradation of BTEX and MTBE. The activation capability was increased with the increase of the amount of slags. To reduce the remediation cost and lessen the Fe (III) precipitation commonly occurred in the groundwater remediation site, BF and BOF slag were predominantly selected as activated agents in replacing Fe (II). Moreover, the MTBE degraded by-products (TBF and TBA) were also effectively degraded under activated conditions by BF and BOF slags. The higher oxygen released rate was observed using PVA/alginate-based hydrogel-encapsulated CaO2 freezing method. Oxygen was also consistently released with the addition of buffering material (citric acid). The oxygen-releasing rates were increased with the decrease of the volumetric ratio of binding material, which is attributed to due to the better oxygen transfer under less amount of binding material condition. DGGE analysis suggested that the microbial community in the PRB system acclimated by BTEX became simplified and approached to certain particular microorganisms. Microbial community structure changes were observed under transient shock organic loading conditions. However, microbial community gradually recovered to its simplified structure when system operated in normal conditions. The SEM photographs show that PVA/alginate beads were suitable for the immobilization of microbial cells. The photograph also indicated that microorganisms could be successfully entrapped inside the pores with homogenous distribution in the PVA/alginate beads. High sensitivity of Vibrio fischeri light inhibition test as a biotoxicity indicator for BTEX detection was observed. The relationship between BTEX concentrations and light inhibition rates was significantly positive. The application of 1% of persufate with 1 and 5 g/L BOF effectively reduced the toxicity of groundwater samples. However, the application of high dose of persulfate (5%) induced the high toxicity. Both immobilized beads-BTEX degrader column and PRB system were proved to effectively degrade the groundwater pollutants, thereby decreasing their biotoxicity. "