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年度	106	專案性質	實驗性質
專案類別	研究專案	研究主題	整治
申請機構	國立中央大學	申請系所	生命科學系
專案主持人	陳師慶	職等/職稱	教授
專案中文名稱	開發加強式生物刺激還原法整治鉻汙染地下水
中文關鍵字	鉻,生物復育,廢水
專案英文名稱	Application of Bioremediation for reduction of Cr(6+) in wastewater
英文關鍵字	Cr,Bioremediation,wasterwater
執行金額	799,480元
執行期間	2017/1/9 至 2017/11/30
計畫中文摘要	工業發展過程中需大量使用鉻重金屬，但由於管理不慎與偷排廢水等情形，導致土壤與地下水鉻汙染層出不窮，環境中鉻常以三價鉻 (Cr3+)和六價鉻 (Cr6+)形式存在，六價鉻則對人體具有高毒性與高致癌性，生物復育法是利用生物材料，例如細菌、真菌或酵素使環境中汙染物轉變成低毒性或無毒性的方法，許多環境中微生物帶有六價鉻還原能力，這些微生物體內酵素能將高毒性的六價鉻還原成三價鉻，一般微生物降解污染物速率通常是非常緩慢的，因此利用適合的方式強化微生物處理汙染物的能力是值得研究的議題。目前加強式生物復育法可分為兩大類，一、生物刺激法 (Biostimulation)；二、生物強化法 (Bioaugmentation)。期中報告部分研究執行內容與成果顯示，使用生物刺激法添加NB作為營養物質可促進現地地下水微生物生長，增加六價鉻還原效率，此外，將生物強化法結合生物刺激法可大幅增加六價鉻還原效率，使整體整治時間降低並達到更好的整治成效。次世代定序菌相分析結果顯示鉻污染地下水經整治處理後鉻還原相關菌屬Bacillus、Acinetobacter和Delftia等比例大幅增加，由此結果推測本研究使用之整治策略可使地下水中鉻還原相關微生物大量增生，加速六價鉻還原，未來可將本研究成果應用於現地模場試驗，以推廣生物整治技術應用。 關鍵字: 鉻、生物刺激法、生物加強法、次世代定序、地下水
計畫英文摘要	Chromium is a heavy metal which is used in large quantities in the process of industrial development. Due to the bad management and illegally discharge of waste water, more and more soil and groundwater are contaminated by the chromium. In the environment, chromium usually is dissociated into trivalent and hexavalent chromium ions, the latter one is highly toxic and highly carcinogenic to the human body. Bioremediation is a waste management technique that involves the use of organisms to neutralize pollutants from a contaminated site. Many microorganisms have an ability to reduce the hexavalent chromium ions into the low toxic trivalent form by the self-express enzymes. Using microorganisms to neutralize pollutants is mild and environmental friendly, so that, it is worthy of study to find a proper way to enhance microbial treatment. In our report, adding NB medium for biostimulation can promote the local groundwater microbial growth, in hence, improving the hexavalent chromium reduce efficiency. Besides, bioaugmentation combined with biostimulation can also increase largely hexavalent chromium reduce efficiency, therefore, the overall remediation time is down and achieve to better remediation effectiveness. The results of Next Generation Sequencing show that ratio of Bacillus, Acinetobacter and Delftia in chromium contaminated groundwater increased after NB medium treat. In the future, this research result can be applied to in-situ bioremediation to promote the application of bioremediation technology. Keywords: Chromium, Biostimulation, Bioaugmentation, Next Generation Sequencing, Groundwater