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年度	112	專案性質	實驗性質
專案類別	先導專案	研究主題	整治
申請機構	慈濟大學	申請系所	醫學檢驗生物技術學系
專案主持人	尤仁音	職等/職稱	教授
專案中文名稱	提升持久性有機污染物快速篩選平台於生物整治模場試驗先行評估計畫
中文關鍵字	持久性有機污染物,快速篩選,異生素反應元件
專案英文名稱	Enhancement of the Rapid Screening Platform for Persistent Organic Pollutants (POPs) in the Pilot Assessment Program for Bioremediation Model Trials
英文關鍵字	Persistent Organic Pollutants, Rapid Screening, Xenobiotic Reactive Element
執行金額	450,000元
執行期間	2024/1/1 至 2024/12/31
計畫中文摘要	各類持久性有機污染物對各層級的生物都構成威脅，由於對環境和生物體都有重大影響，會在高濃度下生物放大，並在食物鏈最高層級的活生物體中累積，在全球各地循環，使得遠程傳播的機率提高，對於源頭管控而言是很大的困難，在避免其進入食物鏈造成生物危害的威脅，更是難以評估。本研究開發能提升芳香烴受體下游的異生素反應元件(XRE)以及缺氧誘導反應元件兩個反應元件檢測感度，以基因工程改良分析平台，建構芳香烴受體-異生素反應元件活性報導系統，應用於持久性有機污染物的篩選，並且運用球型培養傳遞報導系統，發展生物反應模擬以及預測平台，用以評估環境樣本影響之效應。更透過分析缺氧誘導因子β與芳香烴受體核轉位子介導的一級與二級代謝基因的 mRNA 表現量，由上述方案，本計畫已經完成的部分包含: (1) 與POPs關聯的AhR轉錄調節活性區域，(2) AhR 轉錄元件與POPs QSAR計算，(3) 一級二級代謝酵素的冷光素酶活性報導系統:啟動子元件改良，(4) 依各細胞的生物接觸決策路徑初測，(5) 在Spheroid 平台計算組織暴露劑量與時間頻率。以達成建立XRE與HRE元件的多冷光素酶之活性報導系統，由建立的兩個反應元件活性報導細胞，剖析 POPs刺激的 DRE/HRE 決策途徑，應用於持久性有機污染物的篩選，並能監測芳香烴受體誘導的下游代謝與解毒等芳香烴受體下游基因的目標。本研究建置往後能協助量化與推定環境污染物對生物受體之暴露劑量推估的平台，進而探討化學物質可被生物有效接觸利用的特性進行較合理的風險評估。
計畫英文摘要	Various types of persistent organic pollutants pose a threat to organisms at all levels. Due to their significant impact on the environment and organisms, they will biomagnify at high concentrations and accumulate in living organisms at the highest level of the food chain. They are present all over the world. Circulation increases the probability of long-distance transmission, which is very difficult for their source control. It is even more difficult to assess the threat of biological hazards caused by it entering the food chain. This research try to develop the detection system which containing the xenobiotic response element (XRE) and the hypoxia-induced response element (HRE) two response elements that can improve sensitivity of downstream of the aryl hydrocarbon receptor, and uses genetic engineering to improve the analysis platform to construct the aryl hydrocarbon receptor-xenobiotic response element. The activity reporting system is applied to the screening of persistent organic pollutants (POPs), and the sphere culture delivery reporting system is used to develop a biological reaction simulation and prediction platform to evaluate the impact of environmental samples. By analyzing the mRNA expression of primary and secondary metabolism genes mediated by hypoxia-inducible factor β and aryl hydrocarbon receptor nuclear translocation, based on the above scheme, the completed parts of this project include: (1) and POPs The associated AhR transcriptional regulatory activity region, (2) AhR transcriptional elements and POPs QSAR calculation, (3) luciferase activity reporting system of primary and secondary metabolic enzymes: improvement of promoter elements, (4) biological contact according to each cell Preliminary test of the decision path, (5) calculate tissue exposure dose and time frequency on the Spheroid platform. In order to achieve the establishment of a multi-luciferase activity reporting system with XRE and HRE elements, the two reaction element activity reporter cells established can use to analyze the XRE/HRE decision-making pathway stimulated by POPs and be applied to the screening of POPs, and can monitor AhR -induced downstream metabolism and detoxification of AhR downstream genes. This study establishes a platform that can help quantify and infer the exposure dose of environmental pollutants to biological receptors in the future, and then explore the characteristics of chemical substances that can be effectively exposed and utilized by organisms to conduct more reasonable risk assessments.