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年度	105	專案性質	實驗性質
專案類別	研究專案	研究主題	整治
申請機構	國立嘉義大學	申請系所	森林暨自然資源學系
專案主持人	林瑞進	職等/職稱	助理教授
專案中文名稱	木麻黃植物 (Casuarina spp.) 根部共生微生物對於重金屬累積之影響
中文關鍵字	木麻黃植物,放線根瘤菌,暗色隔膜內生菌,菌根菌, 重金屬累積
專案英文名稱	Effects of heavy metal accumulation by different symbiotic endophytes in Casuarina plants
英文關鍵字	Casuarina plants, actinorhizal nodule bacteria, dark septate endophyte, mycorrhizal fungi, heavy metal accumulation
執行金額	727,980元
執行期間	2015/11/17 至 2016/11/16
計畫中文摘要	重金屬 (heavy metal) 為最具警示性及毒性之無機物質，造成土地大面積之污染，而植生復育 (phytoremediation)為利用植物與微生物之共生及相關之技術，木麻黃科 (Casuarinaceae) 為臺灣海岸地區主要造林樹種之一，可與土壤中多種微生物進行共生，可降低環境逆境，幫助植物生長。本研究嘗試釐清接種不同類型之共生微生物是否影響木麻黃植物重金屬吸收量。研究結果顯示，木麻黃植物 (Casuarina equisetifolia)能與三種類型微生物共生，包括叢枝菌根菌 (arbuscular mycorrhizal fungi,AMF)、暗色隔膜內生菌 (dark septateendophytes, DSE) 及固氮放線菌(nitrogen-fixing actinobacteria, NFB)。7株暗色隔膜內生菌分別進行重金屬耐受試驗，3 株菌株 (CkDB5、Rk1 及RrtHH10) 具有耐受1 mg/mL ZnCl2 濃度處理之能力。CkDB5 菌株進一步進行0-3.2 mg/mL ZnCl2 濃度處理，試驗結果顯示ZnCl2 對於菌株完全抑制濃度為2.4 mg/mL，於1.6 mg/mL 濃度處理時，菌絲Zn 含量可達約156.9 mg/g，具有高度之重金屬Zn 吸附能力。以0-4mg/mL ZnCl2 水溶液進行木麻黃苗木重金屬吸收試驗，並探討微生物之影響。試驗結果顯示AMF、DSE 及NFB 幫助木麻黃苗木耐受Zn 之能力，並降低植體內重金屬Zn 含量，但卻能增加植物生長量，進而增加整體重金屬累積量並提升植生復育之效益。
計畫英文摘要	Heavy metals are the most alarming and the most toxic inorganic substances which have contaminated a large area of land. Phytoremediation can be defined as an in situ remediation strategy that uses vegetation and associated microbiota to remove environmental contaminants or render them harmless. Casuarinaceae plants which are one of the main reproducing trees in the eastern coast of Taiwan are able to be associated with different symbiotic soil microorganisms that mitigate the adverse effect of stress and improve plant performance in environments. We try to figure out the effects of heavy metal accumulation by different symbiotic microorganisms in Casuarina plants. The study result demonstrates that Casuarina plants can symbiosis with three kinds of microorganisms including arbuscular mycorrhizal fungi (AMF), dark septate endophytes (DSE) and nitrogen-fixing actinobacteria (NFB). Seven strains of DSE separately test the tolerance capability of heavy metals. The three strains (CkDB5, Rk1 and RrtHH10) show the tolerance capability in 1 mg/mL ZnCl2 concentration. CkDB5 strains further test the tolerance capability by 0-3.2 mg/mL ZnCl2 concentration treadments. The minimum inhibitory concentration (MIC) values of ZnCl2 to CkDB5 strains were about 2.4 mg/mL. In 1.6 mg/mL ZnCl2 treadment, the Zn accumulations of mycelium were about 156.9 mg/g. The CkDB5 strains have highly ability to accumulate Zn in mycelium. In the experiment of heavy metal uptake by Casuarina seedlings via 0-4 mg/mL ZnCl2 solutions as irrigating water, and figure out the effects of microorganisms. The result demonstrates that AMF, DSE and NFB enhance the tolerance ability of Zn in Casuarina seedlings, and reduced the Zn accumulations in plant. But they can promote the growth of plant and then enhance the heavy metal accumulations in total.