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年度	111	專案性質	實驗性質
專案類別	先導專案	研究主題	整治
申請機構	東吳大學	申請系所	微生物學系
專案主持人	張怡塘	職等/職稱	特聘教授兼理學院院長
專案中文名稱	應用底泥微生物同時去除地下水重金屬並生產奈米金屬
中文關鍵字	底泥微生物, 重金屬, 生物合成奈米金屬
專案英文名稱	Simultaneous removal of heavy metals from groundwater and production of nanometals using microorganism in river sediment
英文關鍵字	heavy metals, nanometals
執行金額	410,000元
執行期間	2022/7/1 至 2023/6/30
計畫中文摘要	微生物製作金屬奈米顆粒 (nano particles, NPs) 製作成本低且對環境友善，符合當今綠色化學及低碳足跡產品之要求。本研發專案以循環經濟為構想，建立重金屬循環型資源新技術。首先篩選受重金屬污染河川底泥細菌做為 NPs 製造工廠，成功選出可耐受銅、鋅之菌株。這些菌株產生之胞外聚合物 (extracellular polymeric substance, EPS) 可吸附廢水中之銅、鋅，並符合Freundlich Equation 等溫吸附模式。將吸附重金屬後之菌液透過萃取、濃縮、淨化、封端等步驟，產生胞內奈米氧化銅 (CuO-NPs) 及奈米氧化鋅顆粒 (ZnO-NPs)。實驗結果顯示，篩選耐受金屬菌株對含重金屬銅廢水去除率為76.36-79.49%；對含重金屬鋅廢水之去除率為26.35-33.16%。菌株同時產出符合奈米尺度 (100nm) 之胞內 NPs，產率 (NPs-mg/bacterial cell dry weight-mg) 最高為 Cu4 菌株 (Klebsiella pneumonia，菌種鑑定率100%) 為0.755；Zn3B 菌株 (Bacillus thuringiensis，菌種鑑定率 100%) 為0.271。雖受限於顆粒尺度及純度，胞內 CuO/ZnO-NPs 具有催化能力及抑菌能力等特性，其中 Cu3 菌株生產之 CuO-NP 催化能力為 0.106 min-1及對 E coil (BCRC 11848) 之抑菌能力為76.82% (2.5μg/mL)；Zn3B 菌株生產之 ZnO-NP 催化能力為 0.108 min-1及對 E coil 之抑菌能力為71.65% (2.5 μg/mL)。本專案技術可促進廢水中重金屬之循環，有效降低重金屬進入土壤、地下水等環境汙染，並同時生產胞內NPs，推動金屬資源回收與再利用，符合聯合國SDGs永續發展指標，促進綠色經濟，確保永續消費及生產模式。
計畫英文摘要	The production of nanoparticles (NPs) metals by microorganisms is a low-cost and environment-friendly process, which conforms to a requirement of green chemistry and low carbon footprint products. This project takes in the view of circular economy as a major concept and establishes a new technology for recycling heavy metal from waste. Firstly, bacterial strains from river sediment contaminated by heavy metals were selected as NPs production plants. These strains that can tolerate high amounts of copper and zinc were successfully isolated. The extracellular polymer substrates (EPS) produced by these strains adsorbs copper and zinc in artificial industrial wastewater and conforms to the Freundlich isotherm model. The metals-absorbed bacterial solution goes through the following steps: extraction, concentration, purification, and capping to produce intracellular nano-copper oxide (CuO-NPs) and nano-zinc oxide (ZnO-NPs) particles. The results showed that these metal-tolerant bacterial strains had removal rates of 76.36-79.49% for copper-containing wastewater; removal rates of 26.35-33.16% for zinc-containing wastewater. Nanoscale (100nm) NPs also can be produced. Calculate the NPs yield (NPs-mg/bacterial cell dry weight-mg), the highest rate of production is 0.755 of strain Cu4 (Klebsiella pneumonia, strain identification 100%); strain Zn3B (Bacillus thuringiensis, strain identification 100%) is 0.271. Although these intracellular NPs are limited by particle size and purity, their characteristics are not as good as those of commercially available NPs particles. Intracellular NPs are still presented abilities of high oxidation and antibacterial reaction. Among them, Cu3 strain is calculated as 0.106 min-1 and 76.82% (2.5 μg CuO-NPs/mL) respectively; Zn3B strain is calculated as 0.108 min-1 and 71.65% (2.5 μg ZnO-NPs/mL). This project has successfully developed a technology that promotes the recycling of heavy metals in heavy-metal wastes. A strategy is effective in that recycling and reuse of high-value heavy metals such as copper and zinc, which complies with the UN Sustainable Development Index (SDGs) 12: promoting a green economy and ensuring sustainable consumption and production.