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年度	103	專案性質	實驗性質
專案類別	研究專案	研究主題	整治
申請機構	朝陽科技大學	申請系所	環境工程與管理系所
專案主持人	劉敏信	職等/職稱	副教授
專案中文名稱	利用微生物呼吸試驗探討生物通氣法對柴油污染土壤復育之成效
中文關鍵字	土壤；柴油類碳氫化合物；生物通氣法；微生物呼吸試驗；氣泡式呼吸儀
專案英文名稱	Using Soil Microbial Respiration to Evaluate the Efficiency of Bioventing on Diesel Contaminated Soil
英文關鍵字	Soil；Total Petroleum Hydrocarbon as Diesel；Bioventing；Microbial Respiration；Bubble Respirometer
執行金額
執行期間	2013/11/25 至 2014/11/24
計畫中文摘要	本計畫於實驗室建立高濃度(約 20,000 mg/kg)柴油污染土壤風力驅動式 生物通氣(Wind-driven Bioventing) 模場，使用之土壤經質地分析為砂質壤土， 適合以生物通氣設備進行污染整治，試驗中同時使用氣泡式呼吸儀 (Bubble Respirometer)搭配生物泥漿法進行土壤中微生物活性監測，探討生物通氣法整 治柴油污染土壤的成效與微生物活性之關係。經模場添加具柴油降解能力之 三種微生物及適量營養鹽後，各通氣組皆呈現降解趨勢。Bacillus subtilis 菌 (BC)及 Pseudomonas putida 菌 (PS)於試驗 3 日後之污染物濃度殘餘率 (C/C0) 約為 0.65，而 Achromobacter xylosoxidans 菌 (F3B)僅約為 0.80。經過試驗 30 日後，三種微生物混菌組 (ALL)促使污染物濃度大幅降低，污染物濃度殘餘 率可達 0.30，相較其餘組別有最佳的降解趨勢。以氣泡式呼吸儀於降解期間 對各組別進行呼吸試驗及監測，將每批次試驗開始 24 小時間攝氧率統計作圖 可得一活性曲線，藉由曲線變化能協助操作人員評估微生物活性趨勢。由呼 吸試驗結果顯示，生物通氣模場自營養鹽添加後，BC 組及 PS 組的微生物活 性可連續提高 2 週、F3B 組活性可連續提高 3 週、ALL 組活性則能連續提高 4 週後才逐漸降低。對照攝氧率活性曲線與柴油濃度變化圖後可發現，兩者在 曲線中能呈現一定程度的相關性，顯示攝氧率活性曲線應能作為微生物降解 柴油污染物過程中成效的評估方式。
計畫英文摘要	In this study, a wind-driven bioventing pilot for the high-concentration (about 20,000 mg/kg) diesel contaminated soil was established in the laboratory. According to the analysis, the soil adopted belongs to the sandy loam, which is suitable for the contamination remediation by using the bioventing equipment. During the experiment, a bubble respirometer complete with the bioslurry method was used to monitor the microbial activity in soil, in order to explore the relationship between the effectiveness of the diesel contaminated soil remediation with the bioventing method and the microbial activity. All venting groups presented the degradation tendency after three kinds of microorganisms having the diesel degradation ability and appropriate amount of nutrient sources were added in the pilot. The remaining ratio of diesel concentration (C/C0) of Bacillus subtilis (BC) and Pseudomonas putida (PS) was about 0.65 after 3 days of experiment and that of Achromobacter xylosoxidans (F3B) was only about 0.80. After 30 days of experiment, the mixed of the three kinds of microorganisms (ALL) resulted in the significant reduction of the contaminant concentration and the remaining ratio of contaminant concentration able to reach 0.30, which is the optimal degradation tendency compared with the rest of individual microorganism. The monitoring of microorganisms respiration were carried out with a bubble respirometer on each microorganism during the diesel degradation. An activity curve was plotted by summarizing and mapping the oxygen uptake rates during the first 24 hours of each batch of experiments. Using the curve variance, the microbial activity tendency can be assessed. The respiration tests showed that after the addition of the nutrient sources in the bioventing pilot, the microbial activity of BC and PS increased continuously for two weeks; the activity of F3B increased continuously for three weeks; and the activity of ALL only decreased gradually after four weeks of continuous increase. Comparison of the oxygen uptake rate activity curve and the diesel degradation tendency showed that both can present a certain degree of correlation in the curves, and it is shown that the oxygen uptake rate activity curve can be used as the effectiveness assessment method in the process of diesel contaminant degradation by the microorganisms.