成果專區 - 土壤及地下水污染整治基金補助研究與模場專案宣導網

專案基本資料

年度	103	專案性質	實驗性質
專案類別	模場試驗	研究主題	整治
申請機構	國立中央大學	申請系所	生命科學系
專案主持人	陳師慶	職等/職稱	教授
專案中文名稱	地下水中含氯有機污染物厭氧生物整治
中文關鍵字
專案英文名稱	Anaerobic groundwater bioremediation in chlorinated solvent contaminated site
英文關鍵字	Bioremediation, chloroethene, Metagenome, qPCR
執行金額
執行期間	2014/2/24 至 2014/11/24
計畫中文摘要	氯化有機溶劑常用於工業之清洗溶劑其具有低可燃、易爆、低沸點及高蒸氣壓等特性，如四氯乙烯（perchloroethene, PCE）、三氯乙烯（trichloroethene, TCE）、1,1,2-三氯乙烷（1,1,2-trichloroethane, 1,1,2-TCA）與四氯甲烷（carbon tetrachloride, CT）等。早期台灣區域土壤及地下水污染防治觀念尚未成熟，導致人為的不當傾倒或是意外洩漏使得氯化有機溶劑造成地下水污染。生物整治法被喻為自然、環保及節能的處理技術，也是目前環境污染整治的趨勢。評估現地生物復育技術可行性其中最重要條件之一為場址本身須具有可降解污染物之微生物。當場址中可降解污染物微生物數量較低或場址整治急迫性高時，可額外添加微生物進行生物強化(Bioaugmentation)整治受污染之場址。受污染之場址皆可能含有可降解污染物之微生物存在，若能於污染降解途徑完整之場址確實鑑定出含氯污染物降解途徑中各階段之脫鹵球菌之種類及數量，將可有助於後續場址現地生物整治設計 (採用生物刺激 (Biostimulation)或生物強化)或作為未來生物強化之菌種純化來源。在期末報告的研究執行內容與成果，主要是著重於評估臺南市永康鹽行段設置一模場進行含氯污染物整治試驗，本模場定期採樣偵測結果顯示有監測到含氯污染物降解之趨勢，並產生最終產物乙烯，顯示該模場降解機制完整。模場含氯污染物之降解乃為微生物進行厭氧還原脫氯機制所致。因此本計畫採用 Metagenomics 技術結合 qPCR 進行菌相分析，欲建立完整環境微生物在含氯污染場址之完整生化代謝圖譜，期末成果著重在生物營養鹽添加後是否促進氯化有機汙染物降解，並建立完整環境菌相及降解機制分析，這些成果將應用在未來不同的多氯有機汙染物汙染場址的生物復育研究暨菌相分析工作，作為未來場址全場整治之參考依據亦可作為菌種純化場址之參考來源地點。
計畫英文摘要	The chlorinated solvent (PCE, TCE, 1.2.2-TAC, and CT) has ignitable, explosive, low-melting, and high pressure characteristics, and are often used for cleansing solvent in industries. In the past, we did not realize the issue how we can prevent our dwelling environments near soils or groundwater from contamination, thus leading to the accidental leakage of the chlorinated solvent into the groundwater. Bioremediation is a friendly, cost-effective, and safe technique which is commonly used for remediation in contaminated sites. Bioaugmentation is to add exogenous microorganisms with the potential to degrade polluted chemicals. In this study, we hope to detect the presence of one dehalococcus species with the ability for degrading the chlorinated solvent, and then harvest it for application of bioaugmentation. In the mid-term report, our research field trial is located in 臺南市永康鹽行段 decreased to the undetected level the monitoring well, we indicated that the degradation of the chlorinated solvent occurs. To assure the success of bioremediation in our field trial, we need to confirm the presence of Dehalococoides for future application. In this study, we will first apply the metagenome and qPCR to detect the change of bacterial community before and after bioremediation, and prove the role of Dehalococoides in field trial contaminated with the chlorinated solvent. We herein emphasize that the advantages of metagenome over other molecular techniques are to prove more information about the population of each bacterial genus and even in species within the soils or water, and also to be a fast, and high accurate technique. With this bioremediation proposal, we hope that our biomediation strategies can prove the standard model for the degradation of the chlorinated solvent in field trial.

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