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年度	104	專案性質	實驗性質
專案類別	研究專案	研究主題	調查
申請機構	屏東科技大學	申請系所	環境工程與科學系
專案主持人	趙浩然	職等/職稱	教授
專案中文名稱	發展三維螢光光譜技術追蹤污染場址污染來源之可行性
中文關鍵字	三維螢光光譜, ATP冷光分析, 環境法醫
專案英文名稱	Feasibility of developing the three-dimensional excitation-emission-matrix fluorescence technique to trace the sources of environmental contaminants in the highly contaminated site.
英文關鍵字	Three-dimensional excitation-emission-matrix fluorescence,ATP luciferase analysis, Environment forensic
執行金額	700,000元
執行期間	2014/12/1 至 2015/11/30
計畫中文摘要	近年來，為了落實環境污染稽查工作與預防環境污染事件之發生，發展更 新穎之環境法醫技術釐清污染責任有其必要性。三維螢光光譜具有很高的 敏感性與專一性，可以由此複雜的列陣確認污染物來源。該方法具有高選 擇性、樣品使用量少、快速而準確等特性，非常適合應用於實地受地下水 與土壤污染之場址。本研究結合三維螢光光譜、ATP 冷光分析與化學分析 方法，評估桃園地區受三氯乙烯(Trichloroethylene, TCE)與順-1, 2-二氯乙烯 (cis 1,2-Dichloroethane, c-DCE)之場址污染概況。本次研究結果顯示，24 件 次地下水樣本中 TCE 之分析結果普遍高於地下水污染管制標準，且在深度 15m 與 55m 測得較高之 TCE 濃度。地下水樣本中 DOC 濃度、UV-Vis 光 譜分析與螢光光譜分析結果顯示，類黃酸與腐植酸螢光強度與 TCE 與 c-DCE 濃度呈現顯著正相關性，可藉由評估上述兩項參數來追蹤 TCE 與 c-DCE 之污染程度。
計畫英文摘要	In the recent year, the importance of developing a new environment forensic technique for inspecting sources of environment pollution and in monitoring the changes of environmental contaminants has been the focus of many studies. Due to high sensitivity, specificity, accuracy, rapidity, and it only requires small volume of sample, the three-dimensional excitation-emission-matrix fluorescence technique for qualitative analysis of aromatic compounds is used to confirm the fingerprint of contamination by complicated mixture of aromatic compounds. Therefore, this method may be suitable in tracing the contaminant sources in groundwater and soil. In this study, we combine the three-dimensional excitation-emission-matrix fluorescence technique, ATP luciferase analysis, and chemical analysis to evaluate the trichloroethene (TCE) and cis-1,2-dichloroethene (c-DCE) contaminants’ distribution in a highly contaminated ground water site. The concentrations of TCE in 54 groundwater samples detected in this study were higher than the groundwater pollution control standard, and the highest concentrations were detected at a depth of 15m and 55m. Levels of DOC, UV-Vis and fluorescence in 216 groundwater samples were detected by three-dimensional excitation-emission-matrix fluorescence technique. Two fluorescence parameters (FLF fluorescence level and humic acid fluorescence level) were found to have a positive correlation with TCE and c-DCE. In the future, these two parameters may be important in evaluating and tracing the contaminant sources in groundwater.